Danum Valley is located at approximately one hundred m a. s. l. in Sabah and represents one of many major remaining patches of Sabahs major lowland rainforest. It’s a common equatorial rainforest climate having a mean an nual temperature of 27 C and also a yearly rainfall of 2700 mm. We studied parabioses of Crematogaster modiglianii and Camponotus rufifemur, which nest in hollow tree trunks, Camponotus rufifemur occurs in two chemically and genetically distinct types, which likely represent distinctive species, In con trast, the profiles from the linked Cr. modiglianii had al most no compounds in common with Ca. rufifemur and didn’t display differentiation into chemical varieties, with two exceptions. 27 MeC39 14 ene and 27 MeC39 16 ene, the primary surface elements of your red Ca. rufifemur, occurred in these Cr. modiglianii colonies that lived in parabiosis together with the red Ca.
rufifemur but was absent from many others, Behavioural experiments have been conducted selleck CX-4945 with two nests and one worker colony that have been brought to your laboratory at Danum Valley, wherever both species were stored collectively inside a area of their ori ginal nest trunk in a Fluon covered plastic box for ca. two months or number of days, respectively. For that experiments, additional ants had been collected from 4 added nests, Right here, we use the term nest for entire nests within a liv ing tree trunk, while worker colony refers to groups of employees that had been caught at a parabiotic nest. In accordance for the Ca. rufifemur wide range, the parabiotic colonies will probably be labelled B or R plus a digit in the following. Chemical analyses We analysed the novel compounds from Cr.
modiglianii cuticular extracts working with electron ionization mass spectrom etry, chemical ionization mass spectrometry, higher resolution mass spectrometry, and nuclear magnetic resonance, Furthermore, quite a few de rivatizations had been performed and subsequently analysed with GC MS, First of all, we characterized distinctive substances and their relative quantities selelck kinase inhibitor from seven Cr. modiglianii colonies by their electron ionization mass spec tra. Extracts had been obtained from 20 90 individuals per ex tract, and we analysed one 8 extracts per colony. We performed capillary gasoline chromatography mass spectrom etry having a Hewlett Packard 6890 series gasoline chro matograph coupled to a HP 5973 Mass Selective Detector. The GC was equipped using a J W Scientific DB five fused silica capillary column, The temperature in the GC was stored at 60 C for 2 min, then greater by 60 C min as much as 200 C and subsequently by 4 C min to 320 C, the place it remained constant for ten min. The transfer line had a temperature of 325 C. He lium was made use of as carrier gas using a consistent flow of one. 0 ml min. A split splitless injector was installed at 250 C inside the splitless mode for 30 s.