Electrospray mass spectroscopy was done on fungal taxol samples u

Electrospray mass spectroscopy was done on fungal taxol samples using the electrospray technique with

an Agilent 1100 LC/MSD trap. The sample in 100% methanol was injected with a spray flow of 2 μl/min and a spray voltage of 2.2 kV by the loop injection method. The mass spectral fragment ions of taxol are shown in Table 2. learn more Nucleotide sequence accession numbers The partial sequences of the ITS rDNA, ts, and bapt genes obtained from cultures and clones were deposited in GenBank (NCBI) under the accession numbers JQ801635-JQ801669 and KC337343-KC337345. Acknowledgements This work was supported by the National Basic Research Program of China (973 Program, grant no. 2012CB721104), the National Natural Selleck SB-715992 Science Foundation of China (grants no. 31170101 and 31100073), and the major Projects of Knowledge Innovation Program

of Chinese Academy of Sciences (grant no. KSCX2-EW-J-12). References 1. Kusari S, Spiteller M: Are we ready for industrial production of bioactive plant secondary metabolites utilizing endophytes? Nat Prod Rep 2011, 28:1203–1207.PubMedCrossRef 2. Kusari S, Lamshoft M, Zuhlke S, Spiteller M: An endophytic FK228 fungus from Hypericum perforatum that produces hypericin. J Nat Prod 2008, 71:159–162.PubMedCrossRef 3. Zhu D, Wang J, Zeng Q, Zhang Z, Yan R: A novel endophytic Huperzine A-producing fungus, Shiraia sp. Slf14, isolated from Huperzia serrata . J Appl Microbiol 2010, 109:1469–1478.PubMedCrossRef 4. Stierle A, Strobel G, Stierle D: Taxol and taxane production by Taxomyces andreanae , an endophytic fungus of Pacific yew. Science 1993, 260:214–216.PubMedCrossRef 5. Zhou X, Zhu H, Liu L, Lin J, Tang K: A review: recent advances and future prospects of taxol-producing endophytic fungi. PAK5 Appl Microbiol Biotechnol 2010, 86:1707–1717.PubMedCrossRef 6. Pezzuto J: Taxol production in plant cell culture comes of age. Nat Biotechnol 1996, 14:1083.PubMedCrossRef 7. Nicolaou KC, Yang

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