…) have been selected. All gym and fitness users performing aerobic activities (such as aerobic, spinning, step, circuit training, endurance and cardiovascular

programs, etc.…) were excluded. On the basis of these inclusion/exclusion criteria, a total of 354 participants were retained for the present investigation. this website These subjects were consequently compared with those from our previous study (207 participants), carried out in gyms located in Palermo City (CC) [16]. Questionnaire procedure In order to evaluate the frequency consumption of {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| protein supplements amongst participants, dietary behaviours and other related information, the questionnaire method was adopted [13] (Additional file 1). The same questionnaire has been administered in commercial gyms of the suburbs of Palermo, Italy. Easy understandable definitions of the supplements were provided to the participants (Common and commercial names of products or substances included within the definition of supplement: product intended to supplement LBH589 mw the diet that contains one or more dietary ingredients) [26]. Completion of the questionnaire implied the agreement of respective gym users to participate in the study. According to the Italian regulations, ethical

approval was not required for this study. The same investigator using the face-to-face interview method during a period of six months administered the questionnaire. Food classification Foods were categorized in accordance

to their protein content in three categories: Low, medium and high. We considered low content foods with ≤ 10 g of proteins for 100 g of Fossariinae food, medium those with a protein content between 10 and 20 g every 100 g and finally, high content foods with 20-25 g or above accordingly. The protein content percentage of each food was retrieved from the INRAN database (Istituto Nazionale di Ricerca per gli Alimenti e la Nutrizione; Website: http://​nut.​entecra.​it/​646/​tabelle_​di_​composizione_​degli_​alimenti.​html). Data analysis Data analysis was performed using the EpiInfo software version 7.0 (CDC, Atlanta, GA, US) and Statistica version 8.0 software for Windows (Tulsa, OK, US). The descriptive analysis was performed by calculating the means and standard deviations. Contingency tables were used to assess frequency distribution of protein consumption solely or stratified by gender, frequency of use and food. Differences were assessed by a two-way ANOVA test and a Bonferroni post-hoc test to compare replicate means by row. The associations between the categorical variables under examination were evaluated using contingency tables. Statistical significance was set at P values ≤ 0.05. Results Power analysis showed a statistical power of 0.99 and an effect size of 0.6. Demographic results 561 questionnaires were analysed after the completion of the investigation. Gender stratification has showed 434 male and 137 female participants.