In conclusion, nanotechnology is a highly promising technology that can enhance the safety and therapeutic efficacy of antimicrobials against many intracellular infections. It is critical that the physicochemical properties like particle size, composition, charge, and surface area be appropriately controlled to direct them to specific locations in the body. In addition, biocompatibility and

subcellular delivery of nanostructure may ease the clinical translation. “
“The most frequent cause of bacteraemia among Gram-negative bacteria is Escherichia coli. Analysis of the genes encoding the Shigella enterotoxin 1 (ShET-1), ShET-2, enteroaggregative heat stable MK-2206 price toxin 1 (EAST-1) toxins and AggR factor in E. coli strains causing bacteraemia revealed that set1 genes were presented significantly more frequently among quinolone-susceptible strains (P<0.0001), in phylogenetic group B2 (P=0.0004) and in biofilm strains (P=0.02). In contrast, sen genes were significantly more frequent among nalidixic acid-resistant isolates (15% vs. 6%, P=0.046) and in phylogenetic group B1 (P=0.0001). This is the PI3K inhibitor first study in which ShET1, ShET2 and EAST-1 have been found in E. coli collected from blood. The most frequent cause of bacteraemia among Gram-negative bacteria is Escherichia coli. These isolates possess specialized virulence factors (VFs)

such as adhesins, toxins, iron-acquisition Farnesyltransferase systems, polysaccharide coats and invasines that are not present in commensal and intestinal pathogenic strains (Sannes et al., 2004). The Shigella enterotoxin 1 (ShET-1) toxin

has been described in Shigella flexneri 2a. This toxin is encoded by chromosomal set genes, and these genes have been found on the antisense strand of a mucinase gene in S. flexneri, as well as in enteroaggregative E. coli (EAEC) (Vila et al., 2000; Henderson & Nataro, 2001). The active toxin of ShET-1 has a configuration of one A subunit and several B subunits (A1−Bn) (Noriega et al., 1995; Vargas et al., 1999; Niyogi et al., 2004). The set1 genes are located in the she pathogenicity island (PAI). This PAI is a 46 kb chromosomal element that carries a number of genes with established or potential roles in bacterial virulence (Al-Hasani et al., 2001). In addition to set genes, this PAI includes the sigA gene, which encodes a cytopathic autotransporter protein that contributes to fluid accumulation in ligated rabbit ileal loops (Al-Hasani et al., 2000) and also contains the pic gene (originally she gene), which encodes an autotransporter protein that cleaves mucin and complement and plays a role in inflammation (Henderson & Nataro, 2001). This PAI has been detected in other diarrhoeal pathogens such as Yersinia enterocolitica, Salmonella typhimurium and pathogenic strains of E. coli (Al-Hasani et al., 2001), but has not been sought in E. coli associated with bacteraemia.