The disease has been known in the Indian sub-continent for over a century (Crawford, 1912 and Husain and Nath, 1927). In the United States, HLB is now established in Florida and has resulted in substantial economic losses, estimated to be about US$3.6 billion in economic activity, in a 5 year period (Hodges and Spreen, 2012). Because of the significant financial Dabrafenib concentration implications associated

with HLB, the citrus industries and the regulatory agencies in USA, Brazil, and other countries, are interested in early, rapid detection of the pathogen and subsequent management strategies required to mitigate the disease. Three fastidious gram negative bacteria have been associated with citrus HLB: ‘Candidatus Liberibacter asiaticus’ (Las), ‘Candidatus Liberibacter americanus’ (Lam) and ‘Candidatus Liberibacter africanus’ (Laf). Las is the most prevalent HLB-associated bacterium in Asia as well as in the Western hemisphere. Asian citrus psyllid (ACP; Diaphorina citri Kuwayama), the vector of Las has been reported from most citrus growing regions. The first report of ACP in the United

States was from Florida in 1998 ( Halbert et al., 2000). In Brazil, the psyllid vector prevailed for about 60 years without Cyclopamine mouse the pathogen and did not cause significant damage to the citrus industry ( Bové, 2006 and Lima, 1942). Suggested actions for mitigation of citrus HLB include: a) planting of disease-free nursery stock, b) constant scouting for visual detection of symptomatic trees and subsequent removal and, c) Silibinin control of psyllid vector by pesticide sprays (Belasque et al., 2010, Bové, 2006, Grafton-Cardwell et al., 2013 and Hall et al., 2013). Starting a citrus grove with HLB-tested disease-free nursery stock is an excellent method of disease control and is currently being implemented by regulatory agencies in the United States and Brazil. Reduction of inoculum by removing infected plants based on visual detection of HLB symptoms was followed in many citrus industries including

Brazil (Belasque et al., 2010 and Bové, 2006). It has been shown that infected plants can remain non-symptomatic for an extended period of time, and hence tree removal will not be very effective since the pathogen is known to have a lengthy incubation and latent period (Chiyaka et al., 2012 and Gottwald, 2010). In several locations in Florida, Las was first recorded in psyllids and the subsequent detection in field plants was verified 6 months to 3 years after the initial find in psyllids (Manjunath et al., 2008). Under controlled conditions, Pelz-Stelinski et al. (2010) have demonstrated that it may take one year or longer to detect Las in plants that are successfully inoculated by Las-positive D. citri. HLB disease management based on constant monitoring of the psyllids for Las may be a suitable approach.