DE-AC52-07NA27344, and Los Alamos selleck bio National Laboratory under contract No. DE-AC02-06NA25396.
A representative genomic 16S rRNA sequence of F. taffensis RW262T was compared using NCBI BLAST [10] under default settings (e.g., considering only the high-scoring segment pairs (HSPs) from the best 250 hits) with the most recent release of the Greengenes database [11] and the relative frequencies of taxa and keywords (reduced to their stem [12]) were determined, weighted by BLAST scores. The most frequently occurring genera were Brumimicrobium (62.9%) and Fluviicola (37.1%) (3 hits in total). Among all other species, the one yielding the highest score was ‘Brumimicrobium mesophilum’ (“type”:”entrez-nucleotide”,”attrs”:”text”:”DQ660382″,”term_id”:”110083955″,”term_text”:”DQ660382″DQ660382), which corresponded to an identity of 92.

1% and an HSP coverage of 58.0%. (Note that the Greengenes database uses the INSDC (= EMBL/NCBI/DDBJ) annotation, which is not an authoritative source for nomenclature or classification.) The most frequently occurring keywords within the labels of all environmental samples which yielded hits were ‘lake’ (9.1%), ‘tin’ (3.4%), ‘microbi’ (2.5%), ‘depth’ (2.0%) and ‘tract’ (1.7%) (247 hits in total). The most frequently occurring keywords within those labels of environmental samples which yielded hits of a higher score than the highest scoring species were ‘lake’ (9.2%), ‘tin’ (3.8%), ‘microbi’ (2.3%), ‘depth’ (2.0%) and ‘tract’ (1.8%) (169 hits in total).

The most frequent keyword ‘lake’ may reflect the freshwater origin of strain RW262T, whereas the keywords ‘tin’ and ‘depth’ may allude to some until now unrecognized ecological features of F. taffensis. Figure 1 shows the phylogenetic neighborhood of F. taffensis in a 16S rRNA based tree. The sequences of the two identical 16S rRNA gene copies in the genome differ by two nucleotides from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AF493694″,”term_id”:”62184633″,”term_text”:”AF493694″AF493694), which contains one ambiguous base call. Figure 1 Phylogenetic tree highlighting the position of F. taffensis relative to the type strains of the other species within the family Cryomorphaceae. The tree was inferred from 1,429 aligned characters [13,14] of the 16S rRNA gene sequence under the maximum … Table 1 Classification and general features of F.

taffensis RW262T according to the MIGS recommendations [20] and the NamesforLife database [21]. Strain RW262T is strictly aerobic, Gram-negative, motile by gliding and flexirubin-pigmented [1]. Cells are flexible rods with rounded ends (Figure 2), 0.4-0.5 ��m in diameter and 1.5-5.7 ��m in length, Carfilzomib with rare longer filaments of up to 51 ��m in length [1]. Growth occurs at 4oC and 20oC, but not in the presence of Na+ ions [1]. Growth of strain RW262T at 4 oC is only weak, so that F.